How do sterile bags ensure sterility during storage and transportation to prevent microbial contamination?

In the biomedical industry, how do sterile bags ensure the sterility of cell culture fluids, biological reagents and other sensitive biological products during storage and transportation to prevent microbial contamination?

In the biomedical industry, sterile bags ensure the sterility of cell culture fluids, biological reagents, and other sensitive biological products during storage and transportation to prevent microbial contamination by:

Material selection: Sterile bags are usually made of high-quality, proven materials that have good barrier properties against microorganisms. These materials prevent microorganisms from entering the bag through penetration or diffusion.
Sterilization treatment: Aseptic bags will undergo strict sterilization during the production process, such as ETO ethylene oxide sterilization, gamma cobalt 60 irradiation sterilization, etc., to ensure that the bag itself does not contain any microorganisms.

Sealing performance: The sealing performance of sterile bags is key. High-quality sealing technology can ensure that the bag will not break or leak due to changes in external conditions (such as pressure, temperature, etc.) during storage and transportation, thereby preventing the entry of microorganisms.

Aseptic operation: When placing cell culture fluid, biological reagents and other items into sterile bags, strict aseptic operation procedures must be followed to prevent microbial contamination. This includes performing procedures in a sterile environment, using sterile tools and equipment, etc.

Packaging inspection: After the sterile bag packaging is completed, a series of inspections will be carried out, including appearance inspection, sealing test, sterility verification, etc., to ensure that the bag meets the sterility requirements.

Transportation and storage: During transportation and storage, conditions such as temperature and humidity need to be controlled to maintain the stability of the environment inside the sterile bag. In addition, physical damage to the sterile bag, such as tearing, crushing, etc., needs to be avoided.

Sterile bags ensure the safety of cells during storage and transportation by selecting appropriate materials, performing strict sterilization processes, ensuring good sealing performance, following sterile operating procedures, conducting packaging inspections, and controlling transportation and storage conditions. Sterility of culture fluids, biological reagents and other sensitive biological products to prevent microbial contamination.